Shikimate Hydroxycinnamoyl Transferase (HCT) Activity Assays in Populus nigra
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چکیده
منابع مشابه
Production of coumaroylserotonin and feruloylserotonin in transgenic rice expressing pepper hydroxycinnamoyl-coenzyme A:serotonin N-(hydroxycinnamoyl)transferase.
Transgenic rice (Oryza sativa) plants were engineered to express a N-(hydroxycinnamoyl)transferase from pepper (Capsicum annuum), which has been shown to have hydroxycinnamoyl-CoA:tyramine N-(hydroxycinnamoyl)transferase activity, a key enzyme in the synthesis of hydroxycinnamic acid amides, under the control of constitutive maize (Zea mays) ubiquitin promoter. The transgenic rice plants requir...
متن کاملRed clover HCT2, a hydroxycinnamoyl-coenzyme A:malate hydroxycinnamoyl transferase, plays a crucial role in biosynthesis of phaselic acid and other hydroxycinnamoyl-malate esters in vivo.
In red clover (Trifolium pratense) leaves, phaselic acid (2-O-caffeoyl-L-malate) accumulates to several mmol kg(-1) fresh weight and is a crucial component of a natural system that prevents protein breakdown during harvest and storage of this forage crop. Previously, we identified HCT2, a red clover gene encoding a hydroxycinnamoyl-Coenzyme A (CoA) hydroxycinnamoyl transferase capable of transf...
متن کاملResponses of Populus deltoides × Populus nigra ( Populus × euramericana ) clone I - 214 to high zinc concentrations
• We hypothesized that poplar can be useful for the extraction of zinc (Zn) from contaminated soils and water. However, the physiological response and the base of tolerance of poplar plants to this heavy metal are little known. • Plants of Populus deltoides × P . nigra ( P. × euramericana ) I-214 clone were grown in glasshouse and treated with three different Zn concentrations (1, 100 and 1000 ...
متن کاملSilencing of hydroxycinnamoyl-coenzyme A shikimate/quinate hydroxycinnamoyltransferase affects phenylpropanoid biosynthesis.
The hydroxyl group in the 3-position of the phenylpropanoid compounds is introduced at the level of coumarate shikimate/quinate esters, whose synthesis implicates an acyltransferase activity. Specific antibodies raised against the recombinant tobacco (Nicotiana tabacum) acyltransferase revealed the accumulation of the enzyme in stem vascular tissues of tobacco, in accordance with a putative rol...
متن کاملFlavonoid accumulation in Arabidopsis repressed in lignin synthesis affects auxin transport and plant growth.
In Arabidopsis thaliana, silencing of hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT), a lignin biosynthetic gene, results in a strong reduction of plant growth. We show that, in HCT-silenced plants, lignin synthesis repression leads to the redirection of the metabolic flux into flavonoids through chalcone synthase activity. Several flavonol glycosides and acylated ant...
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2013
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.978